Auto iDeal ChIP-seq kit for Histones
This product must be used with the IP-Star Compact Automated System.
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior to Next-Generation Sequencing. Our expertise in ChIP-seq tools allows reproducible and efficient results every time.
- Proven: Our expertise with ChIP-seq tools enables reproducible and efficient results every time
- Complete: Kit includes control antibodies, control primer pairs and IPure DNA purification module
-
C01010058Auto iDeal ChIP-seq Kit for Transcription Factors
-
C15410003H3K4me3 Antibody
-
C15410195
H3K27me3 Antibody
-
C15410196
H3K27ac Antibody
-
C15410193
H3K9me3 Antibody
Characteristics
Figure 1. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent™ PGM™ (Life Technologies) and GAIIx (Illumina®)
ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 µg of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina®) and PGM™ (Ion Torrent™). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.
Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar
Figure 2. Efficient and easy chromatin shearing using the Bioruptor® and Shearing buffer iS1 from the iDeal ChIP-seq kit
Chromatin from 1 million of Hela cells was sheared using the Bioruptor® combined with the Bioruptor® Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds “ON” / 30 seconds “OFF” at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4°C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.
Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode’s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers
Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.
Species, cell lines, tissues tested
The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.
Cell lines:
Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231
Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2
Hamster: CHO
Tissues
Bee – brain
Daphnia – whole animal
Horse – brain, heart, lamina, liver, lung, skeletal muscles, ovary
Human – Erwing sarcoma tumor samples
Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? Let us know!
Additional solutions compatible with Auto iDeal ChIP-seq Kit for Histones
Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones) optimizes chromatin shearing, a critical step for ChIP.
The MicroPlex Library Preparation Kit provides easy and optimal library preparation of ChIPed samples.
ChIP-seq grade anti-histone antibodies provide high yields with excellent specificity and sensitivity.
Documents
| iDeal ChIP-seq Kit for Histones manual MANUAL The iDeal ChIP-seq Kit for Histones has been validated on IP-Star® Compact Automated System. ... | Download |
Safety sheets
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS GB en | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS US en | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS ES es | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS JP ja | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS DE de | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS BE nl | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS FR fr | Download |
| Auto iDeal ChIP-seq Kit for Histones x24 x100 SDS BE fr | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: Auto iDeal ChIP-seq kit for Histones (Hologic Diagenode Cat# C01010171). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
K27M in canonical and noncanonical H3 variants occurs in distinctoligodendroglial cell lineages in brain midline gliomas. |
Effects of GSK-J4 on JMJD3 Histone Demethylase in Mouse Prostate Cancer Xenografts |
Sp1-Induced SETDB1 Overexpression Transcriptionally InhibitsHPGD in a β-Catenin-Dependent Manner and Promotes theProliferation and Metastasis of Gastric Cancer |
Epigenetic Drifts during Long-Term Intestinal OrganoidCulture |
Chromatin dysregulation associated with NSD1 mutation in head and necksquamous cell carcinoma. |
Postoperative abdominal sepsis induces selective and persistent changes inCTCF binding within the MHC-II region of human monocytes. |
Histone H3.3G34-Mutant Interneuron Progenitors Co-opt PDGFRA for Gliomagenesis. |
EZH2 and KDM6B Expressions Are Associated with Specific EpigeneticSignatures during EMT in Non Small Cell Lung Carcinomas. |
Digging Deeper into Breast Cancer Epigenetics: Insights from ChemicalInhibition of Histone Acetyltransferase TIP60 . |
Human papillomavirus type 38 alters wild-type p53 activity to promote cellproliferation via the downregulation of integrin alpha 1 expression. |
The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape. |
Pervasive H3K27 Acetylation Leads to ERV Expression and a Therapeutic Vulnerability in H3K27M Gliomas. |
H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis. |
PRC2 targeting is a therapeutic strategy for EZ score defined high-risk multiple myeloma patients and overcome resistance to IMiDs. |
SIRT1-dependent epigenetic regulation of H3 and H4 histone acetylation in human breast cancer |
TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors |
Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells |
H4K5 histone acetylation of BRG1 is associated with heroin administration rather than addiction |