Auto iDeal ChIP-seq Kit for Transcription Factors

• Confidence in results: Validated for ChIP-seq with multiple transcription factors
• Proven: Validated by the epigenetics community, including the BLUEPRINT consortium
• Most complete kit available for highest quality data - includes control antibodies and primers
• Validated with Diagenode's MicroPlex Library Preparation™ kit and IP-Star^® Automation System

ChIP-seq on cells

Figure 1. (A) Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina^® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the GAPDH positive control gene.

Figure 1B. The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

Figure 2. Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade HDAC1 (A), LSD1 (B) and p53 antibody (C). The IP'd DNA was subsequently analysed on an Illumina^® Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in regions of chromosome 3 (A), chromosome 12 (B) and chromosome 6 (C) respectively.

ChIP-seq on tissue

Figure 3A. Chromatin Immunoprecipitation has been performed using chromatin from mouse liver tissue, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the Vwf positive control gene.

Figure 3B. The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

The iDeal ChIP-seq Kit for Transcription Factors is compatible with a broad variety of cell lines, tissues and species, as shown below. Other species / cell lines / tissues can be used with this kit.

Cell lines:

Human: A549, A673, BT-549, CD4 T, HCC1806, HeLa, HepG2, HFF, HK-GFP-MR, ILC, K562, KYSE-180, LapC4, M14, MCF7, MDA-MB-231, MDA-MB-436, RDES, SKNO1, VCaP, U2-OS, ZR-75-1                     

Mouse: ESC, NPCs, BZ, GT1-7, acinar cells, HSPCs, Th2 cells, keratinocytes

Cattle: pbMEC, MAC-T

Tissues:

Mouse: kidney, heart, brain, iris, liver, limbs from E10.5 embryos

Horse: liver, brain, heart, lung, skeletal muscle, lamina, ovary

ChIP on yeast

The iDeal ChIP-seq kit for TF is compatible with yeast samples. Check out our Application Note presenting an optimized detailed protocol for ChIP on yeast.

Did you use the iDeal ChIP-seq for Transcription Factors Kit on other cell line / tissue / species? Let us know!

The Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) is the kit compatible with the iDeal ChIP-seq kit for TF, recommended for the optimization of chromatin shearing, a critical step for ChIP.

ChIP Cross-link Gold should be used in combination with formaldehyde when working with higher order and/or dynamic interactions, for efficient protein-protein fixation.

For library preparation of immunoprecipitated samples we recommend to use the MicroPlex Library Preparation Kit - validated for library preparation from picogram inputs.

ChIP-seq grade antibodies provide high yields with excellent specificity and sensitivity.

Check the list of available Primer pairs designed for high specificity to specific genomic regions.

• 

  C05010012

  MicroPlex Library Prep. Kit v2 (12 indexes) - D...

• 

  C15410210

  CTCF Antibody

• 

  C15410053

  HDAC1 Antibody - replaced by the reference C154...

• 

  C15200211

  p300 Antibody

• 

  C15410083

  p53 Antibody

• 

  C15200004

  Pol II Antibody

• 

  C15410067

  LSD1 Antibody

• 

  C01019027

  ChIP Cross-link Gold