BCL6 polyclonal antibody - Classic

Figure 1. Immunoprecipitation
BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell extract A: 30 μg whole cell extract of BCL6 protein expressing Raji cells B: Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody. The immunoprecipitated BCL6 protein was detected by Western blot with the BCL6 antibody diluted 1:1,000.

Figure 2. Western blot results
Sample: 30 μg of whole cell lysate A: Jurkat B: Raji C: NCI-H929 7.5% SDS PAGE BCL6 antibody diluted 1:1,000

Figure 3. Western blot results
BCL6 antibody detects BCL6 protein by Western blot analysis. A. 30 μg Neuro2A whole cell extract B. 30 μg GL261 whole cell extract C. 30 μg C8D30 whole cell extract D. 30 μg NIH-3T3 whole cell extract E. 30 μg Raw264.7 whole cell extract F. 30 μg C2C12 whole cell extract 7.5 % SDS-PAGE The BCL6 antibody (Cat. No. C15410221) was used at a dilution of 1:1,000

Figure 4. IHC
Immunohistochemical analysis of paraffin-embedded Tonsil, using BCL6 (C15410221) antibody (10 μg/ml).

Figure 5. ChIP
ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.Results are expressed as fold enrichment over the IgG negative control.