Beta-glucosyltransferase

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It has been shown that β-glucosyltransferase can be used to discriminate between 5-mC and 5-hmC bases using glucosyl-sensitive restriction enzyme such as MspI, MspJI or GlaI. β-glucosyltransferase treatment is also a convenient method to check for the specificity during hMeDIP or dot blot since the anti-5hmC antibodies do not recognize glucosylated 5hmC.