BRD2 Antibody
Alternative names: FSRG1, NAT, RING3, RNF3
Polyclonal antibody raised in rabbit against mouse Brd2 (bromodomain containing 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.
| Lot | A355-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2, (1) |
Validation Data
Figure 1. Determination of the antibody titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Brd2 (Cat. No. CS-094-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against Brd2
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Brd2 (Cat. No. CS-094-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet Brd2 CS-094-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
Publications
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