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C/EBP alpha Antibody

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Catalog Number
Format
Order
C15410225-100
100 μl
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Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.

Lot43586
Concentration0.59 μg/μl
Species reactivityHuman
TypePolyclonal
PurityPurified by antigen-affinity chromatography.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 2
Immunoprecipitation 1:100 - 1:500 Fig 1
Western Blotting 1:1,000 Fig 1

* Optimal dilutions/concentrations should be determined by the researcher.

    Validation Data

    C/EBP alpha Antibody ChIP Grade

    Figure 1. C/EBP alpha antibody IP results
    C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell extract A: Control with 3 μg of pre-immune rabbit IgG B: Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (C15410225) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (C15410225) diluted 1:1,000.

    C/EBP alpha Antibody ChIP Grade

    Figure 2. C/EBP alpha antibody ChIP results
    ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter. Results are expressed as fold enrichment over the IgG negative control.

    Target Description

    Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide.

     Applications
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
     Documents
    Datasheet CEBP C15410225 DATASHEET
    Datasheet description    		 Download
    Datasheet C/EBP C15410225 - Lot 40058 DATASHEET
    Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), ...    		 Download
     Publications

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    Therapeutic targeting of EP300/CBP by bromodomain inhibition in hematologic malignancies
    Luciano Nicosia et al.
    CCS1477 (inobrodib) is a potent, selective EP300/CBP bromodomain inhibitor which induces cell-cycle arrest and differentiation in hematologic malignancy model systems. In myeloid leukemia cells, it promotes rapid eviction of EP300/CBP from an enhancer subset marked by strong MYB occupancy and high H3K27 acetylation,...

    SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States
    Cossec Jack-Christophe, Theurillat Ilan, Chica Claudia, Búa Aguín Sabela, Gaume Xavier, Andrieux Alexandra, Iturbide Ane, Jouvion Gregory, Li Han, Bossis Guillaume, Seeler Jacob-Sebastian, Torres-Padilla Maria-Elena, Dejean Anne
    Understanding general principles that safeguard cellular identity should reveal critical insights into common mechanisms underlying specification of varied cell types. Here, we show that SUMO modification acts to stabilize cell fate in a variety of contexts. Hyposumoylation enhances pluripotency reprogramming in vit...

    Modulation of vitamin D signaling by the pioneer factor CEBPA.
    Nurminen V, Neme A, Seuter S, Carlberg C
    The myeloid master regulator CCAAT enhancer-binding protein alpha (CEBPA) is known as a pioneer factor. In this study, we report the CEBPA cistrome of THP-1 human monocytes after stimulation with the vitamin D receptor (VDR) ligand 1α,25-dihydroxyvitamin D (1,25(OH)D) for 2, 8 and 24 h. About a third of the ge...