ChIPmentation Kit for Histones
ChIPmentation is a method that combines chromatin immunoprecipiation and tagmentation-based library preparation using a fast and robust ChIP-seq protocol for studying protein/DNA interactions. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters.
The ChIPmentation Kit for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately:
- 24 UDI for Tagmented libraries - Set I, Cat. No. C0101134
- 8 UDI for Tagmented libraries, Cat. No. C0101135
- 24 UDI for Tagmented libraries - Set II, Cat. No. C0101136
- 24 UDI for Tagmented libraries - Set III, Cat. No. C0101137
- 24 SI for Tagmented libraries Cat. No. C01011032
Benefits of the ChIPmentation system for histone ChIP-seq
- Easier and faster than classical ChIP-seq
- Validated for various histone marks for a standard amount of cells
- Generate high quality sequencing data
For low input samples (10,000 cells) we recommend the µChIPmentation kit for Histones.
For ChIP-seq on transcription factors we recommend the iDeal ChIP-seq for transcription factors + TAG kit for ChIPmentation
- 24 UDI forTagmentedlibraries - Set I, Cat. No. C0101134
- 8 UDI forTagmentedlibraries, Cat. No. C0101135
- 24 UDI for Tagmented libraries - Set II, Cat. No. C0101136
- 24 UDI for Tagmented libraries - Set III, Cat. No. C0101137
- 24 SI forTagmentedlibraries Cat. No. C01011032
-
C01070012-30Tagmentase (Tn5 transposase) - loaded - replace...
-
C01011011µChIPmentation Kit for Histones
Validation
The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.
Figure 1: ChIPmentation sequencing results obtained from decreasing starting amounts of cells.
Chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.
Figure 2: ChIPmentation sequencing results.
Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).
Additional solutions compatible with ChIPmentation Kit for Histones
Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones) optimizes chromatin shearing, a critical step for ChIP.
ChIP-seq grade anti-histone antibodies provide high yields with excellent specificity and sensitivity.
For fast and efficient isolation of magnetic beads we recommend the magnetic racks DiaMag0.2.
Primer indexes for tagmenteted libraries:
The kit ChIPmentation for Histones is validated on the IP-Star Compact System and the corresponding protocol is included in the manual.
Documents
| ChIPmentation for Histones MANUAL Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of lib... | Download |
Safety sheets
| ChIPmentation Kit for Histones SDS GB en | Download |
| ChIPmentation Kit for Histones SDS US en | Download |
| ChIPmentation Kit for Histones SDS ES es | Download |
| ChIPmentation Kit for Histones SDS JP ja | Download |
| ChIPmentation Kit for Histones SDS DE de | Download |
| ChIPmentation Kit for Histones SDS BE nl | Download |
| ChIPmentation Kit for Histones SDS FR fr | Download |
| ChIPmentation Kit for Histones SDS BE fr | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: ChIPmentation Kit for Histones (Hologic Diagenode Cat# C01011009). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Time-dependent chromatin maturation during 3D spheroid culture improves preclinical modeling of non-small cell lung cancer |
Epigenetics and suicide: investigating altered H3K14ac unveiled differential expression in ADORA2A, B4GALT2 and MMP14 |
Rhesus macaques self-curing from a schistosome infection can displaycomplete immunity to challenge |
Niche stiffening compromises hair follicle stem cell potential duringageing by reducing bivalent promoter accessibility. |
A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia |
Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells |
Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development |
Measuring Histone Modifications in the Human Parasite Schistosoma mansoni |