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CRISPR/Cas9 Antibody 4G10

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Catalog Number
Format
Order
C15200216-100
100 μg
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Other format
C15200216-10
10 µg
C15200216-50
50 µg /25 µl



Alternative name: Csn1

Monoclonal antibody raised in mouse against the N-terminus of the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein.

Lot004
Concentration1.4 μg/μl
Species reactivityStreptococcus pyogenes
TypeMonoclonal
PurityProtein G purified monoclonal antibody in PBS containing 0.05 % Na-azide.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
Western Blotting 1:5,000 Fig 1
Immunoprecipitation 5 μg/IP Fig 2
Immunofluorescence 1:400 Fig 3
    Validation data

    CRISPR/Cas9 Antibody for Western blot

    Figure 1. Western blot analysis using the monoclonal antibody directed against CRISPR/Cas9
    Western blot was performed on protein extracts from HEK293T cells transfected with Cas9 using the antibody against CRISPR/Cas9 (cat. No. C15200216), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker is shown on the left, position of the Cas9 protein is indicated on the right.

    CRISPR/Cas9 Antibody validated in IP

    Figure 2. IP using the monoclonal antibody directed against CRISPR/Cas9
    IP was performed on whole cell extracts from HEK293T cells transfected with a Cas9 expression vector (lane 1, 3 and 5), or untransfected cells (lane 2 and 4) using 5 μg of the antibody against CRISPR/Cas9 (cat. No. C15200216, lane 3 and 4) or with an equal amount of IgG, used as a negative control (lane 5). The immunoprecipitated proteins were subsequently analysed by Western blot with the polyclonal Cas9 antibody (Cat. No. C15310258, diluted 1:5,000). Lane 1 and 2 show the result of the input.

    CRISPR/Cas9 Antibody for IF

    Figure 3. Immunofluorescence using the monoclonal antibody directed against CRISPR/Cas9
    HEK293T cells were transiently transfected with a Cas9 expression vector. The cells were fixed with 4% formaldehyde, permeabilized in 0,1% Triton X-100 and blocked in PBS containing 5% BSA. The cells were stained with the Cas9 antibody diluted 1;400 at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (left). Nuclei were counter-stained with DAPI (middle). A merge of the two stainings is shown on the right.

     Testimonials

    When doing CRISPR experiments, it is essential to verify the expression levels of the Cas9 to ensure high-quality results. Diagenode's new CRISPR/Cas9 4G10 antibody is extremely specific, able to recognize different Cas9 fusion proteins (even those used for CRISPRi experiments) and compatible with many biochemical assays, such as immunofluorescence, western blot and immunoprecipitation. Among the different antibodies in the market, Diagenode's 4G10 CRISPR/Cas9 is our antibody of choice.

    Researcher at EPFL, Lausanne, Switzerland
     Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
     Documents
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...    		 Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...    		 Download
    Datasheet CRISPR Cas9 4G10 monoclonal antibody DATASHEET
    Monoclonal antibody raised in mouse against the Cas9 nuclease (CRISPR-associated protein 9).    		 Download
    Accurate QC to optimize CRISPR/Cas9 genome editing specificity POSTER
    The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease res...    		 Download
     Safety sheets
    CRISPR/Cas9 Antibody 4G10 SDS GB en Download
    CRISPR/Cas9 Antibody 4G10 SDS US en Download
    CRISPR/Cas9 Antibody 4G10 SDS JP ja Download
    CRISPR/Cas9 Antibody 4G10 SDS DE de Download
    CRISPR/Cas9 Antibody 4G10 SDS BE nl Download
    CRISPR/Cas9 Antibody 4G10 SDS FR fr Download
    CRISPR/Cas9 Antibody 4G10 SDS BE fr Download
    CRISPR/Cas9 Antibody 4G10 SDS ES es Download
     Publications

    How to properly cite our product/service in your work

    We strongly recommend using this: CRISPR/Cas9 Antibody 4G10 (Hologic Diagenode Cat# C15200216-100 Lot# 004). Click here to copy to clipboard.

    Using our products or services in your publication? Let us know!

    Dissecting the epigenetic regulation of the fetal hemoglobin genes to unravel a novel therapeutic approach for β-hemoglobinopathies
    Amistadi, Simone et al.
    Beta-hemoglobinopathies are severe genetic diseases caused by mutations affecting the production of the adult β-globin chain. The clinical severity is mitigated by the co-inheritance of mutations that reactivate the production of the fetal β-like γ-globin in adults. However, the epigenetic mechan...

    Cis-regulatory interfaces reveal the molecular mechanisms underlying the notochord gene regulatory network of Ciona
    Negrón-Piñeiro L. J. et al.
    Tissue-specific gene expression is fundamental in development and evolution, and is mediated by transcription factors and by the cis-regulatory regions (enhancers) that they control. Transcription factors and their respective tissue-specific enhancers are essential components of gene regulatory networks respons...

    Monitoring autochthonous lung tumors induced by somatic CRISPR geneediting in mice using a secreted luciferase.
    Merle N. et al.
    BACKGROUND: In vivo gene editing of somatic cells with CRISPR nucleases has facilitated the generation of autochthonous mouse tumors, which are initiated by genetic alterations relevant to the human disease and progress along a natural timeline as in patients. However, the long and variable, orthotopic tumor growth ...

    Systematic comparison of CRISPR-based transcriptional activatorsuncovers gene-regulatory features of enhancer-promoter interactions.
    Wang K. et al.
    Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of these platforms, their relative potencies and mechanistic differences are incompletely characterized, particularly at h...

    DNA Methylation Editing by CRISPR-guided Excision of 5-Methylcytosine.
    Devesa-Guerra I, Morales-Ruiz T, Pérez-Roldán J, Parrilla-Doblas JT, Dorado-León M, García-Ortiz MV, Ariza RR, Roldán-Arjona T
    Tools for actively targeted DNA demethylation are required to increase our knowledge about regulation and specific functions of this important epigenetic modification. DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosine (5-meC), which may promote its replication-dependent dilution and/or...

    Guidelines for optimized gene knockout using CRISPR/Cas9
    Campenhout CV et al.
    CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a genome-editing experiment can be substantially impacted by technical parameters and biological considerations. Here, we present ...

    Optimization of CRISPR/Cas9 Delivery to Human Hematopoietic Stem and Progenitor Cells for Therapeutic Genomic Rearrangements.
    Lattanzi A, Meneghini V, Pavani G, Amor F, Ramadier S, Felix T, Antoniani C, Masson C, Alibeu O, Lee C, Porteus MH, Bao G, Amendola M, Mavilio F, Miccio A
    Editing the β-globin locus in hematopoietic stem cells is an alternative therapeutic approach for gene therapy of β-thalassemia and sickle cell disease. Using the CRISPR/Cas9 system, we genetically modified human hematopoietic stem and progenitor cells (HSPCs) to mimic the large rearrangements in the &beta...;

    Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo
    Gandhi S. et al.
    The advent of CRISPR/Cas9 has made genome editing possible in virtually any organism, including those not previously amenable to genetic manipulations. Here, we present an optimization of CRISPR/Cas9 for application to early avian embryos with improved efficiency via a three-fold strategy. First, we employed Cas9 pr...

    Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
    Liang W. et al.
    While CRISPR-based gene knock out in mammalian cells has proven to be very efficient, precise insertion of genetic elements via the cellular homology directed repair (HDR) pathway remains a rate-limiting step to seamless genome editing. Under the conditions described here, we achieved up to 56% targeted integration ...

    CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells
    Susan M. Byrne, George M. Church
    CRISPR/Cas9 nuclease systems can create double-stranded DNA breaks at specific sequences to efficiently and precisely disrupt, excise, mutate, insert, or replace genes. However, human embryonic stem cells and induced pluripotent stem cells (iPSCs) are more difficult to transfect and less resilient to DNA damage than...

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