DNMT1 Antibody
Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI
Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.
| Lot | A386-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:1,000 | Fig 1 |
| Western Blotting | 1:500 | Fig 2 (1) |
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Validation Data
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.
Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.
Target description
DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ELISA Enzyme-linked immunosorbent assay. Read more |
Documents
| Datasheet DNMT1 CS-111-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
Publications
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