DNMT3A Antibody
Alternative names: DNMT3A2, TBRS
Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 92-106.
| Lot | A67-001 |
|---|---|
| Concentration | 0.93 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Western Blotting | 1:2,500 | Fig 2 |
| Immunoprecipitation | 2 μg/IP | Fig 2 |
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Validation Data
Figure 1 Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-085-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-085-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3).
Target description
DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
Documents
| Datasheet DNMT3A pAb-085-050 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
Publications
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