DNMT3A polyclonal antibody - Pioneer
Alternative names: DNMT3A2, TBRS
Polyclonal antibody raised in rabbit against mouse DNMT3 (DNA methyltransferase 3A), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.
| Lot | A388-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:1,000 - 1:10,000 | Fig 1 |
| Western blotting | 1:500 | Fig 2 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. Determination of the titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against mouse DNMT3A (Cat. No. CS-107-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:86,500.
Western blot analysis using the Diagenode antibody directed against DNMT3A
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against mouse DNMT3A (Cat. No. CS-107-100) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target description
DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet DNMT3A CS-107-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: DNMT3A polyclonal antibody - Pioneer (Diagenode Cat# C15310107 Lot# A388-001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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