DNMT3B Antibody
Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB
Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using 3 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.
| Lot | A2343-0040 |
|---|---|
| Concentration | 3.2 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:10,000 | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
| Immunofluorescence | 1:1,000 | Fig 3 |
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5-hydroxymethylcytosine (5-hmC) Antibody (rabbit)
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5-formylcytosine (5-fC) Antibody
Validation Data
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against DNMT3B (Cat. No. C15410218). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:220,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B
Whole cell extracts (25 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against DNMT3B (Cat. No. C15410218) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunofluorescence using the Diagenode antibody directed against DNMT3B
Human HeLa cells were stained with the Diagenode antibody against DNMT3B (Cat. No. C15410218) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Target description
DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
Documents
| Datasheet DNMT3B C15410218 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
| SDS C15410218 DNMT3B Antibody GB en | Download |
| SDS C15410218 DNMT3B Antibody US en | Download |
| SDS C15410218 DNMT3B Antibody BE nl | Download |
| SDS C15410218 DNMT3B Antibody BE fr | Download |
| SDS C15410218 DNMT3B Antibody DE de | Download |
| SDS C15410218 DNMT3B Antibody ES es | Download |
| SDS C15410218 DNMT3B Antibody JP ja | Download |
| SDS C15410218 DNMT3B Antibody FR fr | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: DNMT3B Antibody (Hologic Diagenode Cat# C15410218 Lot# A2343-0040). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
ICF-specific DNMT3B dysfunction interferes with intragenic regulation of mRNA transcription and alternative splicing |