DNMT3B Antibody
Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB
Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.
| Lot | A357-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:500 | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
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Validation Data
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT3B (cat# CS-044-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,500.
Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against DNMT3B (cat# CS-044-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.
Target description
DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet DNMT3B CS-044-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
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