ERR alpha Antibody
Polyclonal antibody raised in rabbit against ERR alpha (estrogen-related receptor alpha), using a KLH-conjugated synthetic peptide.
| Lot | 39764 |
|---|---|
| Concentration | 0.7 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 3 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 1 |
| Western Blotting | 1:500 - 1:3,000 | Fig 2, 4, 5 |
Validation Data
Figure 1. IP
ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody. The immunoprecipitated ERR alpha protein was detected by western blot with the ERR alpha antibody (Cat. No. C15410230) diluted 1:1,000.
Figure 2. Western blot
Sample: 30 μg of whole cell lysate A: 293T B: A431 C: H1299 12% SDS PAGE C15410230 diluted 1:500
Figure 3. ERR alpha antibody ChIP results
ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or ERR alpha antibody. The precipitated DNA was detected by PCR with primer set, targeting to PS2 promoter, used as a positive control or GAPDH promoter, used as a negative control.
Figure 4. ERR alpha antibody WB results
ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg mouse kidney extract 10 % SDS-PAGE ERR alpha antibody (cat. No. C15410230) dilution 1:500
Figure 5. ERR alpha antibody WB results
ERR alpha antibody detects ERR alpha protein by western blot analysis. 50 μg rat kidney extract 10 % SDS-PAGE ERR alpha antibody (Cat. No. C15410230) dilution 1:500
Applications
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet ERR C15410230 DATASHEET Datasheet description | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Publications
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