EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.
Exosomes are small extracellular vesicules of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.
Exosomes contain various molecular constituents of their cell of origin, including proteins and microRNA. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.
Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.
EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples.
The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.

Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium.
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