FLAG Antibody
Monoclonal antibody raised in mouse against Flag™ (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.
| Lot | 002 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Carboxy and amino terminal linked FLAG™ tagged recombinant proteins |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:20,000 - 1:100,000 | |
| Western Blotting | 1:2,000 - 1:20,000 | Fig 1, 2 |
Validation Data
Figure 1. FLAG antibody western blot results
Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 μL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.
Figure 2. FLAG antibody western blot results
Monoclonal Antibody to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet FLAG C15200209 DATASHEET Datasheet description | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: FLAG Antibody (Hologic Diagenode Cat# C15200209 Lot# 002). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment. |