H3K18me2 Antibody
Polyclonal antibody raised in rabbit against Histone H3 (dimethyl Lys18), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.79 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | Fig 1 |
| Immunofluorescence | 1:500 | Fig 2 |
| Western Blotting | 1:500 | Fig 4, 5 |
| Immunochemistry | 1:100 | |
| Dot Blotting | 1:1,000 | Fig 3 |
Validation Data
Figure 1. ChIP
Chromatin Immunoprecipitation of H3K18me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K18me2 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using real-time PCR and normalized to the input chromatin.
Figure 2. Immunofluorescence
Immunofluorescence of H3K18me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:500 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K18me2 is nuclear and chromosomal. Staining: Histone H3K18me2 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Figure 3. Dot Blot
Dot Blot of H3K18me2 antibody. Lane 1: K18 unmodified. Lane 2: K18Me2. Lane 3: K18Me3. Lane 4: K18ac. Lane 5: K18Me1. Load: 0.1, 0.3, and 1 picomoles of peptide. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Figure 4. Western Blot
Western Blot of H3K18me2 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 5. Western Blot
Western Blot of H3K18me2 antibody. 30 μg of NIH-3T3 histone extract. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Target Description
The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H3K18me2 C15410291 DATASHEET Datasheet description | Download |
Publications
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