H3K27me2/3 monoclonal antibody - Classic
Monoclonal antibody raised in mouse against histone H3, di- or trimethylated at lysine 27 (H3K27me2/3), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.5 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein G purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 1.5 μl | Ref 1 |
| Dot Blotting | 1:300 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
| Immunofluorescence | 1:100 | Ref 1 |
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Validation Data
Figure 1. Cross reactivity of the Diagenode monoclonal antibody directed against H3K27me2/3
A Dot Blot analysis was performed to test the cross reactivity of the Diagenode monoclonal antibody against H3K27me2/3 (cat# MAb-014-050) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.
Figure 2. Western blot analysis using the Diagenode monoclonal antibody against H3K27me2/3
Histone extracts from HeLa cells (HeLa HE, 20 μg) were analysed by Western blot using the Diagenode monoclonal antibody against H3K27me2/3 (cat# MAb-013-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right.
Applications
| DB Dot blotting Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H3K27me2-3 MAb-014-050 DATASHEET Datasheet description | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: H3K27me2/3 monoclonal antibody - Classic (Diagenode Cat# C15200014 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Identification of Novel Molecular Markers of Human Th17 Cells. |