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H3K37me1 Antibody

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Catalog Number
Format
Order
C15410295
50 μg
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Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.42 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1 μg/ml Fig 1
Dot Blotting 1:1,000 Fig 2, 3
    Validation Data

    Western Blot

    Figure 1. Western Blot
    Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot Blot

    Figure 2. Dot Blot
    Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

    Dot Blot

    Figure 3. Dot Blot
    Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

     Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
     Documents
    Datasheet H3K37me1 C15410295 DATASHEET
    Datasheet description    		 Download
     Publications

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    SMYD5 is a histone H3-specific methyltransferase mediatingmono-methylation of histone H3 lysine 36 and 37.
    Aljazi Mohammad B. et al.
    Although post-translational modifications (-PTMs) of some histone H3 lysine residues are well studied, the PTMs of histone H3 lysine 37 in mammalian cells remain largely unknown. In this study, we provide evidence to show that SMYD family member 5 (SMYD5) is a histone H3-specfic methyltransferase that catalyzes mono...