H3K4me1T6p Antibody
Polyclonal antibody raised in rabbit against Histone H3 (monomethyl Lys4, p Thr6), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.55 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | |
| Immunohistochemistry | 1:50 | |
| Immunofluorescence | 1:50 | Fig 1 |
| Western Blotting | 1:500 | Fig 2, 3 |
| Dot Blotting | 1:1,000 | Fig 4 |
Validation Data
Figure 1. H3K4me1T6p antibody Immunofluorescence results
Immunofluorescence of H3K4me1T6p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue).
Figure 2. H3K4me1T6p antibody Western blot results
Western Blot of Rabbit H3K4me1T6p antibody. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 3. H3K4me1T6p antibody Western blot results
Western Blot of H3K4me1T6p antibody. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. H3K4me1T6p antibody Dot blot results
Dot Blot of H3K4me1T6p antibody. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Applications
| IHC Immunohistochemistry Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
Documents
| Datasheet H3K4me1T6p C15410280 DATASHEET Datasheet description | Download |
Publications
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