H3K56me3 Antibody
Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys56), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.71 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | |
| Immunofluorescence | 1:100 | Fig 1, 2 |
| Western Blotting | 1:500 - 1:1,000 | Fig 3 |
| Dot Blotting | 1:40 | Fig 4 |
Validation Data
Figure 1. Immunofluorescence
Immunofluorescence using the H3K56me3 antibody. Tissue: HeLa cells during telophase. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me3 is nuclear and chromosomal. Staining: Histone H3K56me3 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Figure 2. Immunofluorescence
Immunofluorescence using the H3K56me3 antibody. Tissue: HeLa cells during prophase. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me3 is nuclear and chromosomal. Staining: Histone H3K56me3 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Figure 3. Western Blot
Western Blot using the H3K56me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. Dot Blot
Dot Blot using the H3K56me3 antibody. Lane 1: H3K56ac. Lane 2: H3K56me1. Lane 3: H3K56me2. Lane 4: H3K56me3. Lane 5: H3K56 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:40 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
Documents
| Datasheet H3K56me3 C15410297 DATASHEET Datasheet description | Download |
Publications
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