H3R17me2(asym) Antibody
Polyclonal antibody raised in rabbit against Histone H3 (asym-dimethyl Arg17), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.83 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | Fig 1 |
| Immunofluorescence | 1:50 | Fig 2 |
| Western Blotting | 1:500 | Fig 3, 4 |
Validation Data
Figure 1. Chromatin Immunoprecipitation
Chromatin Immunoprecipitation of H3R17me2a antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of H3R17me2a antibody alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.
Figure 2. Immunofluorescence
Immunofluorescence of H3R17me2a antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3R17me2a is nuclear and chromosomal. Staining: Histone H3R17me2a is expressed in green, nuclei are counterstained with Dapi (blue).
Figure 3. Western Blot
Western Blot of H3R17me2a antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. Western Blot
Western Blot of H3R17me2a antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Target Description
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H3R17me2a C15410289 DATASHEET Datasheet description | Download |
Safety sheets
| SDS C15410289 H3R17me2 asym Antibody GB en | Download |
| SDS C15410289 H3R17me2 asym Antibody US en | Download |
| SDS C15410289 H3R17me2 asym Antibody ES es | Download |
| SDS C15410289 H3R17me2 asym Antibody JP ja | Download |
| SDS C15410289 H3R17me2 asym Antibody DE de | Download |
| SDS C15410289 H3R17me2 asym Antibody BE nl | Download |
| SDS C15410289 H3R17me2 asym Antibody FR fr | Download |
| SDS C15410289 H3R17me2 asym Antibody BE fr | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: H3R17me2(asym) Antibody (Hologic Diagenode Cat# C15410289 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |