H3R2me2sT3p Antibody
Polyclonal antibody raised in rabbit against Histone H3 (p Thr3, Sym-dimethyl Arg2), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.21 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Immunofluorescence | 1:50-1:100 | Figure 1 |
| Western Blotting | 1 μg/mL | Figure 2 |
| Dot Blotting | 1:1,000 | Figure 3 |
Validation Data
Figure 1. H3R2me2sT3p Immunofluorescence results
Immunofluorescence of H3R2me2sT3p. The H3R2me2sT3p antibody was tested at a 1:50 dilution in Hela cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Figure 2. H3R2me2sT3p Western blot results
Western Blot of H3R2me2sT3p antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Lane 2: NIH/3T3 cell lysates. Lane 3: Cos 7 cell lysates. Load: 35 μg per lane. Primary antibody used at 1 μg/mL overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: 15 kDa. Other band(s): non-specific.
Figure 3. H3R2me2sT3p Dot blot results
Dot Blot of H3R2me2sT3p antibody. Lane 1: Unmodified. Lane 2: R2me2s. Lane 3: T3p. Lane 4: R2me1. Lane 5: R2me2a. Lane 6: R2me2s. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40,000 for 30 min at RT.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
Documents
| Datasheet H3R2me2sT3p C15410303 DATASHEET Datasheet description | Download |
Publications
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