H3T3pK4ac Antibody
Polyclonal antibody raised in rabbit against Histone H3 (ac Lys4, p Thr3), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.57 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | |
| Immunohistochemistry | 1:50 | |
| Immunofluorescence | 1:100 | Figure 1 |
| Western Blotting | 1:500 | Figure 2 |
| Dot Blotting | 1:1,000 | Figure 3 |
Validation Data
Figure 1. H3T3pK4ac antibody Immunofluorescence results
Immunofluorescence Microscopy of H3T3pK4ac antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T3pK4ac is nuclear and chromosomal. Staining: H3T3pK4ac is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Figure 2. H3T3pK4ac antibody Western blot results
Western Blot of H3T3pK4ac antibody. Lane 1: HeLa histone extracts. Lane 2. NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 3. H3T3pK4ac antibody Dot blot results
Dot Blot of H3T3pK4ac antibody. Lane 1: T3p. Lane 2: T3pK4me1. Lane 3: T3pK4me2. Lane 4: T3pK4ac. Lane 5: T3pR2me2s. Lane 6: K4 unmodified. Lane 7: K4me1. Lane 8: K4me2. Lane 9: K4me3. Lane 10: K4ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Applications
| IHC Immunohistochemistry Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
Documents
| Datasheet H3T3pK4ac C15410279 DATASHEET Datasheet description | Download |
Publications
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