H3T3pK4me1 Antibody
Polyclonal antibody raised in rabbit against Histone H3 (p Thr3, monomethyl Lys4), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.98 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Immunohistochemistry | 1:100 | |
| Immunofluorescence | 1:100 | Figure 1 |
| Western Blotting | 1:1,000 | Figure 2 |
| Dot Blotting | 1:1,000 | Figure 3 |
Validation Data
Figure 1. H3T3pK4me1 antibody Immunofluorescence results
Immunofluorescence Microscopy of H3T3pK4me1 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T3pK4me1 is nuclear and chromosomal. Staining: Histone H3T3pK4me1 is expressed in green while the nuclei and aplpha- tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Figure 2. H3T3pK4me1 antibody Western blot results
Western Blot of Rabbit H3T3pK4me1 antibody. Lane 1: HeLa histone extracts. Lane 2. NIH 3T3 histone extracts Lane 3. C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at a 1:1,000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 3. H3T3pK4me1 antibody Dot blot results
Dot Blot of H3T3pK4me1 antibody. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT.
Applications
| IHC Immunohistochemistry Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
Documents
| Datasheet H3T3pK4me1 C15410277 DATASHEET Datasheet description | Download |
Publications
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