H3T45p Antibody
Polyclonal antibody raised in rabbit against the region of histone H3 containing the phosphorylated threonine 45 (H3T45p), using a KLH-conjugated synthetic peptide.
| Lot | A2280P |
|---|---|
| Concentration | 1.5 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:5,000 - 1:10,000 | Fig 1 |
| Dot Blotting | 1:20,000 | Fig 2 |
Validation data
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3T45p (cat. No. 15410321) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:350,000.
Figure 2. Cross reactivity tests using the Diagenode antibody directed against H3T45p
To test the cross reactivity of the Diagenode antibody against H3T45p (cat. No. 15410321), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3T45. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 2 shows a high specificity of the antibody for the modification of interest.
Documents
| Datasheet H3T45p antibody DATASHEET Datasheet of H3T45p antibody | Download |
Safety sheets
Publications
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