H3T6pK9me2 Antibody
Polyclonal antibody raised in rabbit against Histone H3 (p Thr6, dimethyl Lys9), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.49 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | Figure 1 |
| Immunohistochemistry | 1:100 | |
| Immunofluorescence | 1:100 | |
| Western Blotting | 1:500 |
Validation Data
Figure 1. H3T6pK9me2 antibody ChIP results
Chromatin Immunoprecipitation of H3T6pK9me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me2 antibody to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.
Target Description
The doubly modified dimethyl K9, phospho-T6 histone H3 is known to exist, but very little comprehensive information about the importance of this modification and its mechanism has been published. H3K9me2 is a histone post-translational modification that is enriched in transcriptionally inactive regions. It is also important in cellular differentiation and maturation. Phosphorylation of histone H3 is also associated with mitosis and meiosis, and seems to be related to developmental changes. The dual modification of H3T6pK9me2 is still under investigation and will present more insight into the complex epigenetic code.
Applications
| IHC Immunohistochemistry Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H3T6pK9me2 C15410284 DATASHEET Datasheet description | Download |
Publications
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