H4K12ac Antibody
Monoclonal antibody raised in mouse against histone H4, acetylated at lysine 16 (H4K16ac), using a KLH-conjugated synthetic peptide.
| Lot | 001-11 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 1-2 μg/ChIP | Figure 1 |
| Western Blotting | 1:500 | Figure 2 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
Validation Data
Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K12ac
ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K12ac (Cat. No. C15200218) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Western blot analysis using the Diagenode monoclonal directed antibody against H4K12ac
Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H4K12ac (Cat. No. C15200218) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target Description
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active genes.
Applications
| DB Dot blotting Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| C15200218 H4K12ac monoclonal DATASHEET Datasheet description | Download |
Safety sheets
Publications
How to properly cite our product/service in your workWe strongly recommend using this: H4K12ac Antibody (Hologic Diagenode Cat# C15200218 Lot# 001-11). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
TIP60/P400/H4K12ac Plays a Role as a Heterochromatin Back-up Skeleton inBreast Cancer. |