H4K20me2 Antibody
Polyclonal antibody raised in rabbit against Histone H4 (dimethyl Lys20), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.53 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | Fig 1 |
| Immunofluorescence | 1:50 | Fig 2 |
| Western Blotting | 1:2,000 | Fig 3, 4 |
| Immunochemistry | 1:50 |
Validation Data
Figure 1. ChIP
Chromatin Immunoprecipitation of H4K20me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H4K20me2 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.
Figure 2. Immunofluorescence
Immunofluorescence of H4K20me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4K20me2 is nuclear and chromosomal. Staining: Histone H4K20me2 is expressed in green, nuclei are counterstained with DAPI (blue).
Figure 3. Western Blot
Western Blot of H4K20me2 antibody. 30 μg C. elegans embryo lysate. Primary antibody used at a 1:2000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.
Figure 4. Western Blot
Western Blot of H4K20me2 antibody. 30 μg HeLa histone extracts. Primary antibody at a 1:2000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.
Target Description
When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can seriously affect replication and stability of genomic information.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H4K20me2 C15410301 DATASHEET Datasheet description | Download |
Publications
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