H4K8ac polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against Histone H4 (acetyl Lys8) (histone cluster 1, H4a), using a KLH-conjugated synthetic peptide.
| Lot | 41423 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Figure 1 |
| Dot Blotting | 1:5,000 | Figure 3 |
| ICC/IF | 1:100 - 1:1,000 | Figure 5 |
| IHC | 1:100 - 1:1,000 | Figure 6, 7, 8 |
| Immunoprecipitation | 1:100 - 1:5,000 | Figure 4 |
| Western Blotting | 1:1,000 - 1:10,000 | Figure 2 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. H4K8ac
ChIP results ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or H4K8ac antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Figure 2. H4K8ac WB results
H4K8ac antibody detects Histone H4 acetylated at Lys8 by Western blot analysis.
A. 30 μg HeLa whole extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole extract (0.4 μM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE H4K8ac antibody dilution: 1:5,000
Figure 3. H4K8ac
Dot blot results Dotblot analysis of H4K8ac antibody with different peptides. Varied amount of the peptides were spotted onto positively charged nylon membrane and blotted with the H4K8ac antibody at a 1:5,000 dilution. A: Peptide H4K8un B: Peptide H4K8ac C: Peptide H4K16un D: Peptide H4K16ac
Figure 4. H4K8ac IP results
H4K8ac antibody immunoprecipitates Histone H4 acetylated at Lys8 in IP experiments. IP Sample: HeLa whole cell extract
A: 30 μg whole cell extract of HeLa cells (0.4 uM TSA treatment for 18hr)
B: Control with 2.5 μg of pre-immune rabbit IgG C: Immunoprecipitation of Histone H4 acetylated at Lys8 by 2.5 μg of H4K8ac antibody. The immunoprecipitated Histone H4 (acetyl K8) protein was detected by western blot with the H4K8ac antibody diluted 1:5,000.
Figure 5. H4K8ac IF results
H4K8ac antibody detects Histone H4 acetylated at Lys8 in the nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 acetylated at Lys8 stained by H4K8ac antibody diluted 1:500. Red: alpha Tubulin antibody, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Figure 6. H4K8ac
IHC results H4K8ac antibody detects Histone H4 acetylated at Lys8 at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. H4K8ac antibody dilution: 1:500.
Figure 7. H4K8ac
IHC results H4K8ac antibody detects Histone H4 acetylated at Lys8 at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. H4K8ac antibody dilution: 1:500.
Figure 8. H4K8ac
IHC results H4K8ac antibody detects Histone H4 acetylated at Lys8 at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. H4K8ac antibody dilution: 1:500.
Applications
| DB Dot blotting Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IC Immunochemistry Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H4K8ac C15410235 DATASHEET Datasheet description | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: H4K8ac polyclonal antibody - Classic (Diagenode Cat# C15410235-100 Lot# 41423). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Chronic stress leads to epigenetic dysregulation in the neuropeptide-Y and cannabinoid CB1 receptor genes in the mouse cingulate cortex |