H4R3me1 Antibody
Polyclonal antibody raised in rabbit against Histone H4 (monomethyl Arg3), using a KLH-conjugated synthetic peptide.
| Lot | 002 |
|---|---|
| Concentration | 0.64 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Storage Buffer | 20 mM Potassium phosphate, 150 mM Sodium chloride, 30% glycerol, 0.01% Sodium azide. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | |
| Immunofluorescence | 1:50 | Figure 1 |
| Western Blotting | 1:1,000 | Figure 2 |
| Immunochemistry | 1:50 | |
| Dot Blotting | 1:1,000 |
Validation Data
Figure 1. Immunofluorescence
Immunofluorescence of H4R3me1 antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4R3me1 is nuclear and chromosomal. Staining: Histone H4R3me1 is expressed in green, nuclei are counterstained with DAPI (blue).
Figure 2. Western Blot
Western Blot of H4R3me1 antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:1,000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
| IC Immunochemistry Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H4R3me1 C15410299 DATASHEET Datasheet description | Download |
Publications
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