HA tag Antibody
Monoclonal antibody raised in mouse against the HA tag (amino acid sequence YPYDVPDYA) using a KLH-conjugated synthetic peptide. The HA antibody recognizes the influenza Hemagglutinin epitope which is extensively used as a protein tag in expression vectors. This antibody is extremely specific and allows unambiguous identification and quantitative analysis of HA tagged proteins.
| Lot | 003 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Specific for HA-tagged fusion proteins expressed in bacteria, insect, mammalian cells |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| Western Blotting | 1:1,000 - 1:3,000 | Fig 1 |
| IP/ChIP | 1:150 - 1:200 | |
| IS | 1:500 - 1:1,000 |
* Please note that optimal antibody amount should be determined by the end-user for each specific assay condition.
Validation Data
Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag
Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet HAtag MAb-190-050 DATASHEET Datasheet description | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Safety sheets
Publications
How to properly cite our product/service in your workWe strongly recommend using this: HA tag Antibody (Hologic Diagenode Cat# C15200190 Lot# 003). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
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