HDAC1 Antibody

Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against HDAC1
ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against HDAC1 (Cat. No. C15100144) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (Cat. No. C01010070), using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the coding region of p21, a known target gene of HDAC1. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against HDAC1
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode monoclonal antibody against HDAC1 (Cat. No. C15100144) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.

Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against HDAC1
Whole cell extracts (40 μg) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against HDAC1 (Cat. No. C15100144) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.