HP1α, β & γ antibody
Alternative names: CBX5, 1, 3
Polyclonal antibody raised in rabbit against human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms.
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human, mouse: positive. Other species: not tested |
| Type | Polyclonal |
| Purity | Protein G purified polyclonal antibody |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300 |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 | Fig 1 |
| Immunofluorescence | 1:500 | Fig 2 |
Validation Data
Figure 1. Western blot analysis using the Diagenode antibody directed against HP1α, ß and γ
Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α, ß and γ (Cat. No. C15410071) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1ß and HP1γ is indicated on the right.
Figure 2. Immunofluorescence using the Diagenode antibody directed against HP1α, ß and γ
HeLa cells were stained with the Diagenode antibody against HP1α, ß and γ (Cat. No. C15410071) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, ß and γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
Documents
| Datasheet hHP1 pAb-071-050 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
Publications
How to properly cite our product/service in your workWe strongly recommend using this: HP1α, β & γ antibody (Hologic Diagenode Cat# C15410071 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells. |