Jarid1b Antibody

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Jarid1b (Cat. No. CS-113-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.

Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1b (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Jarid1b (Cat. No. CS-113-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.