JMJD2a Antibody

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2a (Cat. No. pAb-126-050), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2a
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. pAb-126-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.