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L. bacterium CRISPR/Cpf1 Antibody

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Catalog Number
Format
Order
C15200233-100
100 µg
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Other format
C15200233-10
10 µg
C15200233-50
50 µg/50 µl



Monoclonal antibody raised in mouse against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.

Lot001
Concentration1 µg/µl
Species reactivityLachnospiraceae bacterium
TypeMonoclonal
PurityProtein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution/amount References
Western Blotting 1:1,000 - 1:20,000 Fig 1, 2
    Validation data
    WB

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1
    Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1
    Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag.

     Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
     Documents
    L. bacterium CRISPR/Cpf1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Pre...    		 Download
    Accurate QC to optimize CRISPR/Cas9 genome editing specificity POSTER
    The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease res...    		 Download
     Safety sheets
    L.bacterium CRISPR Cpf1 antibody SDS GB en Download
    L.bacterium CRISPR Cpf1 antibody SDS US en Download
    L.bacterium CRISPR Cpf1 antibody SDS ES es Download
    L.bacterium CRISPR Cpf1 antibody SDS JP ja Download
    L.bacterium CRISPR Cpf1 antibody SDS DE de Download
    L.bacterium CRISPR Cpf1 antibody SDS BE nl Download
    L.bacterium CRISPR Cpf1 antibody SDS FR fr Download
    L.bacterium CRISPR Cpf1 antibody SDS BE fr Download
     Publications

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    One-step generation of modular CAR-T cells with AAV-Cpf1.
    Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S
    Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activatin...