Mouse IgG (sample size)
The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses present in serum of healthy animals. This IgG preparation is intended for use as a negative control in ChIP experiments (but also in MeDIP, IF and other experiments) for specific antibodies made in mouse. The negative Ctrl IgG from mouse should be used for ChIP in parallel with specific antibody at the same concentration as the specific antibody.
| Lot | MIG004C |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | none |
| Type | Polyclonal, ChIP-grade, CUT&Tag-grade |
| Purity | Purified by protein A chromatography |
| Host | Mouse |
| Storage Buffer | 2 mM phosphate, 30 mM NaCl, ph 7.8; 0.04% sodium azide. Contains sucrose for stabilization |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Validation data
Figure 1. ChIP with the mouse IgG negative control antibody
ChIP assays were performed on sheared chromatin from 1 million HeLa cells using the mouse monoclonal antibody against H3K27ac (cat. No. C15200184) and the Hologic Diagenode ChIP kit. Mouse IgG (cat. No. C15400001) was used as a negative IP control. One microgram (µg) of each antibody was used per ChIP experiment. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and EIF4A2 genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Cut&Tag results obtained with the rabbit IgG negative control antibody
Cut&Tag was performed on 50,000 K562 cells using 0.5 µg of the rabbit monoclonal antibody against H3K27me3 (cat. No. C15200181) and the Universal Cut&Tag kit (cat. No. C01070024). Mouse IgG (cat. No. C15400001) was used as a negative control. The resulting libraries were analyzed on a Fragment Analyzer. Figure 2 shows the results with the H3K27me3 antibody on top and the IgG negative control at the bottom.
Figure 3. Immunofluorescence with the mouse IgG negative control antibody
HeLa cells were stained with the mouse monoclonal antibody against H3K27ac (cat. No. C15200184) (top) and with DAPI. Mouse IgG (cat. No. C15400001) was used as a negative control (bottom). Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with either the H3K27ac antibody or the mouse IgG negative control antibody (left), each diluted 1:500 in blocking solution; followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Target Description
The negative control IgG from mouse has been extensively validated in chromatin immunoprecipitation (ChIP). It contains a spectrum of the IgG subclasses present in serum of healthy mice. This IgG preparation is intended for use as a negative control in ChIP, MeDIP, IF and other experiments performed with specific antibodies made in mouse. The negative control IgG from mouse should be used in parallel with the specific antibody at the same concentration. It is also included in many of our ChIP and MeDIP kits.
Applications
| Methylated DNA immunoprecipitation Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNARead the method The Methylated DNA Immunoprec... Read more |
| ChIP-qPCR (ab) Read more |
| CUT&Tag The quality of antibody used in CUT&Tag is one of the crucial factors for assay success. The antibodies with confirmed high specificity will target only the protein of interest, enabling real results. Check out our selection of antibodies vali... Read more |
Documents
| Datasheet mouseIgG C15400001 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
Publications
How to properly cite our product/service in your workWe strongly recommend using this: Mouse IgG (sample size) (Hologic Diagenode Cat# C15400001-15 Lot# MIG004C). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Endurance training increases a ubiquitylated form of histone H3 in the skeletal muscle, supporting Notch1 upregulation in an MDM2-dependent manner |
ETV2/ER71 regulates hematovascular lineage generation and vascularization through an H3K9 demethylase, KDM4A |
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2 |
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2 |
The Effect of Metformin and Carbohydrate-Controlled Diet onDNA Methylation and Gene Expression in the Endometrium of Womenwith Polycystic Ovary Syndrome. |
The histone modification H3K4me3 is altered at the locus in Alzheimer'sdisease brain. |
Inhibition of HIV-1 gene transcription by KAP1 in myeloid lineage. |
p53 directly represses human LINE1 transposons. |
Attenuated Epigenetic Suppression of Muscle Stem Cell Necroptosis Is Required for Efficient Regeneration of Dystrophic Muscles. |
Episo: quantitative estimation of RNA 5-methylcytosine at isoform level by high-throughput sequencing of RNA treated with bisulfite. |
Centromeres License the Mitotic Condensation of Yeast Chromosome Arms. |
A novel FOXA1/ESR1 interacting pathway: A study of Oncomine™ breast cancer microarrays |
TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors |
Differentiation of Mouse Enteric Nervous System Progenitor Cells is Controlled by Endothelin 3 and Requires Regulation of Ednrb by SOX10 and ZEB2 |
High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development |
Overexpression of histone demethylase Fbxl10 leads to enhanced migration in mouse embryonic fibroblasts. |
Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse |
Overexpression of caspase 7 is ERα dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip) |
Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses. |
Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo |
The genetic association of RUNX3 with ankylosing spondylitis can be explained by allele-specific effects on IRF4 recruitment that alter gene expression |
ClpX stimulates the mitochondrial unfolded protein response (UPRmt) in mammalian cells |
Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response. |
DNA methylation directs functional maturation of pancreatic β cells |
Trrap-dependent histone acetylation specifically regulates cell-cycle gene transcription to control neural progenitor fate decisions. |
Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. |
Genome-wide analysis of LXRα activation reveals new transcriptional networks in human atherosclerotic foam cells. |
New partners in regulation of gene expression: the enhancer of trithorax and polycomb corto interacts with methylated ribosomal protein l12 via its chromodomain. |
The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology and the metabolome of fibroblasts |