NFKB cRel Antibody
Polyclonal antibody raised in rabbit against NFKB p50 (NFKB1), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:5,000 - 1:25,000 | |
| Gel Shift | 1:500 | |
| Western Blotting | 1:500 - 1:3,000 | Fig 1 |
Validation Data
Figure 1. NFkB cRel antibody western blot results
Western blot of HeLa cell extract. All incubations were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5’ each. The western blot was incubated with secondary antibody (HRP Goat anti Rabbit IgG) diluted 1:2,000 for 1 h.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet NFKB cRel C15310257 DATASHEET Datasheet description | Download |
Safety sheets
| SDS C15310257 NFKB cRel Antibody GB en | Download |
| SDS C15310257 NFKB cRel Antibody US en | Download |
| SDS C15310257 NFKB cRel Antibody DE de | Download |
| SDS C15310257 NFKB cRel Antibody BE nl | Download |
| SDS C15310257 NFKB cRel Antibody FR fr | Download |
| SDS C15310257 NFKB cRel Antibody BE fr | Download |
| SDS C15310257 NFKB cRel Antibody ES es | Download |
| SDS C15310257 NFKB cRel Antibody JP ja | Download |
Publications
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