PPARG Antibody

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PPARG (Cat. No. C15310133). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:27,600.

Figure 2. Western blot analysis using the Diagenode antibody directed against PPARG
293T cells were analysed by Western blot using the Diagenode antibody against PPARG (Cat. No. C15310133) diluted 1:500. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 2) or with an empty vector (lane 1). The position of the protein of interest is indicated on the right (expected size: 58 kDa); the marker (in kDa) is shown on the left.