RARA Antibody
Alternative names: RAR, RARalpha, NR1B1
Polyclonal antibody raised in rabbit against human RARA (Retinoic Acid Receptor alpha) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
| Lot | A704-002 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mice |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 4 μl/ChIP | Fig 1, 2 |
| ELISA | 1:100 - 1:500 | Fig 3 |
| Western Blotting | 1:750 | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.
Validation Data
A. ChIP-seq signals on chromosome 19
B. 50 kb region on chromosome 19 surrounding the HMHA1 gene
C. 50 kb region on chromosome 19 surrounding the PRAM1 gene
Figure 1. ChIP-seq results obtained with the Diagenode antibody directed against RARA
ChIP was performed as described above and the IP’d DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.
Figure 2. ChIP results obtained with the Diagenode antibody directed against RARA
ChIP assays were performed using NB4 cells, the Diagenode antibody against RARA (Cat. No. C15310155) and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human RARA (Cat. No. C15310155). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:11,300.
Figure 4. Western blot analysis using the Diagenode antibody directed against RARA
Human embryonic kidney cells (293T) were transfected with a RARA construct (lane 2) or with a negative control construct (lane 1) and analysed by Western blot using the Diagenode antibody against RARA (Cat. No. C15310155), diluted 1:750 in BSA/PBS- Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Target Description
RARA (UniProtKB/Swiss-Prot entry P10276) is a receptor for retinoic acid, a vitamin A metabolite, which directly regulates gene expression in target cells by binding to specific DNA response elements. In the absence of its ligand, this receptor represses transcription through the recruitment of specific corepressors and of HDAC’s, whereas binding of retinioc acid causes the recruitment of coactivators and HAT’s. Translocations involving the RARA gene, often leading to a RARA/PML fusion protein, are a major cause of acute promyelocytic leukemia.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet RARA CS-155-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
| SDS C15310155 RARA Antibody GB en | Download |
| SDS C15310155 RARA Antibody US en | Download |
| SDS C15310155 RARA Antibody BE nl | Download |
| SDS C15310155 RARA Antibody FR fr | Download |
| SDS C15310155 RARA Antibody BE fr | Download |
| SDS C15310155 RARA Antibody ES es | Download |
| SDS C15310155 RARA Antibody JP ja | Download |
| SDS C15310155 RARA Antibody DE de | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: RARA Antibody (Hologic Diagenode Cat# C15310155 Lot# A704-002). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Retinoic acid and ascorbate synergize to suppress myeloid leukemia via TET2 activation |
ALDH1A1 promotes PARP inhibitor resistance by enhancing retinoic acidreceptor-mediated DNA polymerase θ expression. |
Systems-biology analysis of rheumatoid arthritis fibroblast-likesynoviocytes implicates cell line-specific transcription factor function. |
Short-Chain Fatty Acids Calibrate RARα Activity RegulatingFood Sensitization |
Retinoic Acid Receptor Alpha Represses a Th9 Transcriptional and Epigenomic Program to Reduce Allergic Pathology. |
Human retinoic acid-regulated CD161 regulatory T cells support wound repair in intestinal mucosa. |
Elevated Fibroblast growth factor 21 (FGF21) in obese, insulin resistant states is normalised by the synthetic retinoid Fenretinide in mice |
The oncofusion protein FUS-ERG targets key hematopoietic regulators and modulates the all-trans retinoic acid signaling pathway in t(16;21) acute myeloid leukemia. |
Retinoic acid is essential for Th1 cell lineage stability and prevents transition to a Th17 cell program. |
PML-RARalpha/RXR Alters the Epigenetic Landscape in Acute Promyelocytic Leukemia. |