RbAp48 Antibody
Alterantive names: RBBP4, CAF-1 subunit C, CAF-I P48, NURF55, Lin-53.
Monoclonal antibody raised in mouse against retinoblastoma binding protein 4, using a KLH-conjugated synthetic peptide.
| Lot | 40186 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein G purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| ICC/IF | 1:100 - 1:500 | Fig 3 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:100 - 1:500 | Fig 4 |
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C3001001715 ml Bioruptor® Pico Tubes
Validation Data
Figure 1. ChIP
RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune mouse IgG B. 5 μg of RbAp48 antibody (C15200206) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
Figure 2. Immunoprecipitation
RbAp48 antibody immunoprecipitates RbAp48 protein in IP experiments. IP Sample: 1,000 μg HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2.5 μg of preimmune mouse IgG C. Immunoprecipitation of RbAp48 protein with 2.5 μg of RbAp48 antibody (C15200206) The immunoprecipitated RbAp48 protein was detected by western blot with the RbAp48 antibody diluted 1:500.
Figure 3. Immunofluorescent analysis
Immunofluorescence analysis of HeLa cells, using RbAp48 (Cat. No. C15200206) antibody at a 1:100 dilution.
Figure 4. Western blot
Detection of RbAp48 in HeLa nuclear extract.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet RbAp48 C15200206 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: RbAp48 Antibody (Hologic Diagenode Cat# C15200206 Lot# 40186). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
PKC-mediated phosphorylation of BCL11B at Serine 2 negatively regulates its interaction with NuRD complexes during CD4+ T cell activation |