Ski3 Antibody
Alternative names: TTC37
Polyclonal antibody raised in rabbit against human SKI3, using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 3.0 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Protein G purified |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300 |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:100 | Fig 1 |
| Immunofluorescence | 1:100 | Fig 2, Ref 1 |
| ChIP | 1 - 5 μg/ChIP | Ref 1 |
Validation Data
Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3
Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 µg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. C15410011) diluted 1:100. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left.
Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3
HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus.
Target Description
SKI3 is a component of the SKI complex which is composed of SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, making the link between the SKI complex and the exosome in order to perform mRNA degradation.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet SKI3 pAb-011-150 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
Publications
How to properly cite our product/service in your workWe strongly recommend using this: Ski3 Antibody (Hologic Diagenode Cat# C15410011 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |