SMAD2 Antibody
Polyclonal antibody raised in rabbit against SMAD2, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.55 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100,000 | |
| Western Blotting | 1:500 - 1:3,000 | Fig 1 |
Validation Data
Figure 1. SMAD2 antibody Western blot results
Western blot using the Diagenode antibody against SMAD2 to detect over-expressed SMAD2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of SMAD2 in lysates of COS transfected with SMAD2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGFß. Low levels of staining in control lanes correspond to detection of endogenous SMAD2. The membrane was probed with the primary antibody at a 1:2500 dilution.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet SMAD2 C15410273 DATASHEET Datasheet description | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: SMAD2 Antibody (Hologic Diagenode Cat# C15410273 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
The TGF-β profibrotic cascade targets ecto-5'-nucleotidase gene in proximal tubule epithelial cells and is a traceable marker of progressive diabetic kidney disease. |