SMAD3S423/425p Antibody
Alternative names: MADH3, JV15-2, HMAD-3, HSMAD3, MAD3, LDS3, HSPC193, SMAD 3
Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:2,000 | Fig 1 |
| Immunohistochemistry | 1:400 | Fig 2 |
Validation data
Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p
Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.
Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p
Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).
Target description
Applications
Documents
| SMAD3S423/425p polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser42... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Safety sheets
| SMAD3S423/425p Antibody SDS GB en | Download |
| SMAD3S423/425p Antibody SDS US en | Download |
| SMAD3S423/425p Antibody SDS BE nl | Download |
| SMAD3S423/425p Antibody SDS FR fr | Download |
| SMAD3S423/425p Antibody SDS BE fr | Download |
| SMAD3S423/425p Antibody SDS ES es | Download |
| SMAD3S423/425p Antibody SDS JP ja | Download |
| SMAD3S423/425p Antibody SDS DE de | Download |
Publications
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