SMYD3 Antibody
Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1
Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.
| Lot | 41906 |
|---|---|
| Concentration | 1.32 μg/μl |
| Species reactivity | Human, mouse, rat: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP* | 5 µg/ChIP | Fig 1 |
| Western blotting | 1:500 - 1:1,000 | Fig 2, 3 |
| Immunoprecipitation | 5 µg/IP | Fig 4 |
| IF | 1:500 | Fig 5 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
Validation Data
Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3
ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.
Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3
Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3
Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3
Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.
Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33
HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.
Target Description
SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet SMYD3 C15410253 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: SMYD3 Antibody (Hologic Diagenode Cat# C15410253-100 Lot# 41906). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
SMYD3: a new regulator of the early steps of adipocyte differentiation |