STAT2 Antibody
Alternative names: STAT113, P113, ISGF-3
Monoclonal antibody raised in mouse against STAT2, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.0 μg/μl |
| Species reactivity | Human, mouse |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ELISA | 1:5,000 - 1:10,000 | |
| Western Blotting | 1:1,000 | Fig 1 |
| Immunohistochemistry | 1:2.5 μg/mL | Fig 2 |
* Optimal dilutions/concentrations should be determined by the researcher.
Validation Data
Figure 1. STAT2 western blot results
Western blot was performed on 1 μg HeLa whole cell lysate with the STAT2 monoclonal antibody (Cat. No. C15200210). The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with the Diagenode STAT2 antibody diluted 1:1,000 in 3% BSA/TBST overnight at 4C.
Figure 2. STAT2 antibody immunohistochemistry results
The Diagenode STAT2 monoclonal antibody was used at a 2.5 μg/mL to detect STAT2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (figure 2). Expression of STAT2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IHC Immunohistochemistry Read more |
Documents
| Datasheet STAT2 C15200210 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
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