TDG Antibody
Alternative name: HTDG
Monoclonal antibody raised in mouse against TDG (Thymine-DNA glycosylase), using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Affinity purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 | Fig 1 |
| Immunofluorescence | 1:400 | Fig 2 |
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C15410204-1005-Carboxylcytosine (5-caC) Antibody
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C15310200
5-formylcytosine (5-fC) Antibody
Validation data
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against TDG
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against TDG(Cat. No. C15200227) diluted 1:1,000. The position of the TDG and the sumoylated TDG protein is indicated on the right; the marker (in kDa) is shown on the left.
Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against TDG
HeLa cells were stained with the Diagenode antibody against TDG (Cat. No. C15200227) diluted 1:400 followed by an anti-mouse antibody conjugated to Alexa488 (left). The right figure shows staining of the nuclei with DAPI.
Target description
TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
Documents
| Datasheet TDG monoclonal antibody - C15200227 DATASHEET Monoclonal antibody raised in mouse against TDG (Thymine-DNA glycosylase), using a recombinant pr... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: TDG Antibody (Hologic Diagenode Cat# C15200227 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |