TET2 Antibody

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Catalog Number

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C15200179
(MAb-179-050)

50 μg/50 μl

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Alternative name: MDS
Monoclonal antibody raised in mouse against a recombinant protein containing the N-terminal 300 amino acids of human TET2 (tet oncogene family member 2).

Lot	005
Concentration	1.5 µg/µl
Species reactivity	Human, mouse
Type	Monoclonal
Purity	Protein G purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:1,000 - 1:5,000	Fig 1, 2
Immunoprecipitation	5 μg/mg of RIPA lysate	Fig 2

Validation Data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against TET2
Figure 1A. Whole cell extracts from HeLa cells (40 µg) were analysed by Western blot using the Diagenode antibody against TET2 (cat. No. C15200179), diluted 1:2,000 in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Figure 1B. Western blot on mouse E14 ES cells. The antibody was used at a dilution of 1:1,000.

Figure 2. Immunoprecipitation using the Diagenode monoclonal antibody directed against TET2
IP was performed on 250 µg HL60 RIPA cell lysate using the Diagenode antibody against TET2 (cat. No. C15200179) (lane 3) or an IgG negative control (lane 2). The samples were analysed by Western blot analysis as described above. The input sample (25 µg RIPA lysate) was used as a positive control (lane 1).

Target description

TET2 (UniProt/Swiss-Prot entry Q6N021) is a methylcytosine dioxygenase that catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC). 5-hmC has been recently discovered in mammalian DNA and is abundant in Purkinje neurons, granule cells, embryonic stem cells, and brain tissue, especially in areas that are associated with higher cognitive function. Although its precise role has still to be shown, recent studies indicate that 5-hmC plays important roles distinct from 5-mC. Early evidence suggests that 5-hmC may represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. Mutations in TET2 have been associated with myeloproliferative diseases such as essential thrombocythemia, polycythemia vera and primary myelofibrosis.

Applications

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

IP
Immunoprecipitation Read more

Documents

Datasheet TET2 C15200179 DATASHEET Monoclonal antibody raised in mouse against a recombinant protein containing the N-terminal 300 a...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

Safety sheets

TET2 antibody SDS US en	Download
TET2 antibody SDS GB en	Download
TET2 antibody SDS BE nl	Download
TET2 antibody SDS FR fr	Download
TET2 antibody SDS BE fr	Download
TET2 antibody SDS ES es	Download
TET2 antibody SDS JP ja	Download
TET2 antibody SDS DE de	Download

Publications

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Vitamin C enhances NF-κB-driven epigenomic reprogramming andboosts the immunogenic properties of dendritic cells.
Morante-Palacios O. et al.
Dendritic cells (DCs), the most potent antigen-presenting cells, are necessary for effective activation of naïve T cells. DCs' immunological properties are modulated in response to various stimuli. Active DNA demethylation is crucial for DC differentiation and function. Vitamin C, a known cofactor of ten-eleven...

Coordinated glucocorticoid receptor and MAFB action inducestolerogenesis and epigenome remodeling in dendritic cells
Morante-Palacios Octavio et al.
Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic cells (DCs), central actors for coordinating immune responses, acquire tolerogenic properties in response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment fo...

TET2 inhibits tumorigenesis of breast cancer cells by regulating caspase-4.
Zhu X, Li S
Epigenetic regulators have been shown to influence breast cancer progression. However, the detailed mechanism by which TET2 plays the suppressive role in tumorigenesis remains not completely understood. We employed RT-qPCR and westernblot to examine genes expression. Next, the bisulphite sequencing PCR was used to d...

The Role of N-α-acetyltransferase 10 Protein in DNA Methylation and Genomic Imprinting
Lee C.C. et al.
Genomic imprinting is an allelic gene expression phenomenon primarily controlled by allele-specific DNA methylation at the imprinting control region (ICR), but the underlying mechanism remains largely unclear. N-α-acetyltransferase 10 protein (Naa10p) catalyzes N-α-acetylation of nascent proteins, and mu...

TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells
Garcia-Gomez A. et al.
The plasticity of myeloid cells is illustrated by a diversity of functions including their role as effectors of innate immunity as macrophages (MACs) and bone remodelling as osteoclasts (OCs). TET2, a methylcytosine dioxygenase highly expressed in these cells and frequently mutated in myeloid leukemias, may be a key...

TET2 binds the androgen receptor and loss is associated with prostate cancer
Nickerson M.L. et al.
Genetic alterations associated with prostate cancer (PCa) may be identified by sequencing metastatic tumour genomes to identify molecular markers at this lethal stage of disease. Previously, we characterized somatic alterations in metastatic tumours in the methylcytosine dioxygenase ten-eleven translocation 2 (TET2)...

TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.
Marina RJ et al.
Intragenic 5-methylcytosine and CTCF mediate opposing effects on pre-mRNA splicing: CTCF promotes inclusion of weak upstream exons through RNA polymerase II pausing, whereas 5-methylcytosine evicts CTCF, leading to exon exclusion. However, the mechanisms governing dynamic DNA methylation at CTCF-binding sites were u...

Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.
Neri F, Incarnato D, Krepelova A, Rapelli S, Anselmi F, Parlato C, Medana C, Dal Bello F, Oliviero S
Ten eleven translocation (Tet) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC can be further excised by thymine-DNA glycosylase (Tdg). Here, we present a genome-wide approach, named methylation-assisted bisulfite seq...

TET2 Antibody

How to properly cite our product/service in your work

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