TIP5 Antibody
Alternative names: BAZ2A, WALp3
Polyclonal antibody raised in rabbit against TIP5 (Transcription termination factor I-interacting protein 5), using the recombinant protein.
| Lot | A300-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 5 μl/ChIP | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.
Validation Data
Figure 1. ChIP results obtained with the Diagenode antibody directed against TIP5
ChIP assays were performed using the Diagenode antibody against TIP5 (cat. No. CS-090-100). Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor (Diagenode) to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure 1 shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.
Figure 2. Western blot analysis using the Diagenode antibody directed against TIP5
Western blot was performed on 150 μg nuclear extract from either NIH3T3 or HeLa cells with the Diagenode antibody against TIP5 (cat. No. CS-090-100), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween- 20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.
Target Description
TIP 5 (UniProt/Swiss-Prot entry Q9UIF9) is the large subunit of the nucleolar remodeling complex NoRC. NoRC causes the repression of ribosomal gene transcription. It was demonstrated that histone deacetylation is involved in this repression and that TIP5 is associated with the histone deacetylase HDAC1 and mediates the deacetylation of histones in the vicinity of the rDNA promoter. The interaction of TIP5 and HDAC1, which is necessary for transcriptional repression, is established by the C-terminal PHD finger and bromodomain.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet TIP5 CS-090-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Safety sheets
| SDS C15310090 TIP5 Antibody GB en | Download |
| SDS C15310090 TIP5 Antibody US en | Download |
| SDS C15310090 TIP5 Antibody JP ja | Download |
| SDS C15310090 TIP5 Antibody BE nl | Download |
| SDS C15310090 TIP5 Antibody BE fr | Download |
| SDS C15310090 TIP5 Antibody FR fr | Download |
| SDS C15310090 TIP5 Antibody ES es | Download |
| SDS C15310090 TIP5 Antibody DE de | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: TIP5 Antibody (Hologic Diagenode Cat# C15310090 Lot# A300-004 ). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
The RNA helicase DHX9 establishes nucleolar heterochromatin, and this activity is required for embryonic stem cell differentiation |
ING1 regulates rRNA levels by altering nucleolar chromatin structure and mTOR localization |
BAZ2A (TIP5) is involved in epigenetic alterations in prostate cancer and its overexpression predicts disease recurrence. |
lncRNA Maturation to Initiate Heterochromatin Formation in the Nucleolus Is Required for Exit from Pluripotency in ESCs. |
Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription. |
The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats |
Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z. |
Epigenetic engineering of ribosomal RNA genes enhances protein production. |